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About the Instrument

A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of (or alongside) traditional reflection and absorption to study specimens. It works by using a high-intensity light source (like UV) to excite molecules, which then emit a lower-energy, longer-wavelength light to generate a high-contrast image. 

Measurements & Analysis

• Spatial Analysis: Measures distances, areas, volumes, and velocities of biological structures. It is governed by resolution limits defined by the numerical aperture and emission wavelength.
• Intensity Analysis: Evaluates pixel values to determine relative fluorophore concentrations. The most reliable metric is the Mean Fluorescence Intensity (MFI). 

Applications

• Life Sciences & Biomedical Research
• Clinical & Medical
• Diagnostics
• Forensics & Materials

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