The Metabolomics Division is equipped with several platforms of chromatography and mass spectrometers in addition to an amino acid analyzer. Among the most sensitive instruments is the Triple Q-Trap MS/MS detector, which has enough specificity and sensitivity to identify most small compounds and metabolites. The Division performs annual proficiency testing for newborn screening tests in collaboration with the Center of Disease Control and Prevention (CDC, as well as proficiency testing with the College of American Pathologists (CAP) for amino acid tests, and organic acid tests.

 The Division consists of two laboratories, the Enzymology Lab, and Drug and Metabolite Monitoring Lab. Whereas the Enzymology Lab is specialized in diagnosis of Lysosomal Storage Disorders (LSDs), the Drug and Metabolite Monitoring Lab routinely performs various diagnostic tests for diagnostic tests for Organic Acid and Amino Acid Metabolic Disorders.

The Newborn Screening Project which started in 2009 integrated Quattro micro™ LC-MS/MS instrument in routine testing analysis.  The objectives were to verify the prevalence for inborn errors of 29 metabolic disorders in Jordanian population..  .

The Division provides training for graduate and undergraduate students on gas chromatography (GC) and high performance liquid chromatography (HPLC).