Genomics Research Group

 

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The center has two units of ABI PRISM® 310 Genetic Analyzers purchased from Applied Biosystems, USA, which is an automated single-capillary genetic analyzer designed for a wide range of sequencing and fragment analysis applications including comparative sequencing, linkage analysis, SNP detection, discovery and validation, mutation detection, forensics and many other applications.

 

The Mx4000 system is the world's first instrument designed specifically for multiplexed QPCR applications. The Mx4000 system supports all available fluorescent chemistries and is intended to create a new standard for data analysis and flexibility. Stratagene's Mx4000 multiplex quantitative PCR system is the only instrument designed specifically for multiplexed real- time QPCR assay applications.

 

 

 For PCR amplification, three iCycler (BioRad, USA) PCR machines are used in the center. These machines amplify DNA quantity of multiple samples at once. It has many features like ease of soft ware, temperature gradient and monitoring, and optional security for protection of folders and protocols.

                                                     

                                                    

 

The center has also a Gel Documentation system. This unit is equipped with a PC, darkroom, UV transilluminator, epi-white illumination, camera, PCI digitizing card, cables, and finally  Quantity One software for enhancement and saving of images .

 

 

For primary mutation screening the center is equipped with a DCode Universal Mutation Detection System (BioRad, USA). The purpose of this machine is to identify mutations within specific genes which is an important strategy in the diagnosis and prognosis for many diseases. Several techniques, including DGGE, CDGE, TTGE and SSCP, have been developed to examine heterogeneous tissue samples for specific mutations.

 

 

Nucleic acid separation is routinely done in the center using different types of vertical and horizontal electrophoresis systems (BioRad, USA) for both agarose and polyacrylamide gels.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Facility          People          Protocols       Current Research          Fees       Links